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shewanella halifaxensis haw eb4 shal 1523  (ATCC)
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ATCC shewanella halifaxensis haw eb4 shal 1523
Shewanella Halifaxensis Haw Eb4 Shal 1523, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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probe  (Hamilton Company)
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Hamilton Company probe
Probe, supplied by Hamilton Company, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mrs1523  (MedChemExpress)
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MedChemExpress mrs1523
The effects of AR antagonists on ChEC migration and AKT activation. ( A ) Cell migration was assessed using a Transwell assay in the presence of the vehicle (con), NECA (10 µM), or a combination of NECA with caffeine (400 µM), DPCPX (A 1 AR antagonist, 100 nM), Istradefylline (A 2A AR antagonist, 1 µM), MRS1754 (A 2B AR antagonist, 100 nM), or <t>MRS1523</t> (A 3 AR antagonist, 100 nM). Migration was quantified by counting the number of cells that migrated through the membrane in 8 high-power fields (×200; *** p < 0.001, * p < 0.05, n = 3). ( B ) AKT activation was determined by Western blot analysis using AKT phosphorylation-specific and total protein antibodies after the incubation of ChEC with the vehicle (con), NECA (10 µM), and combinations of NECA with caffeine, DPCPX (A 1 AR antagonist, 100 nM), or Istradefylline (A 2A AR antagonist, 1 µM) for 1 h. ( C ) Band intensities were assessed using the ImageJ software.
Mrs1523, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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strain ncppb 1523  (ATCC)
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ATCC strain ncppb 1523
The effects of AR antagonists on ChEC migration and AKT activation. ( A ) Cell migration was assessed using a Transwell assay in the presence of the vehicle (con), NECA (10 µM), or a combination of NECA with caffeine (400 µM), DPCPX (A 1 AR antagonist, 100 nM), Istradefylline (A 2A AR antagonist, 1 µM), MRS1754 (A 2B AR antagonist, 100 nM), or <t>MRS1523</t> (A 3 AR antagonist, 100 nM). Migration was quantified by counting the number of cells that migrated through the membrane in 8 high-power fields (×200; *** p < 0.001, * p < 0.05, n = 3). ( B ) AKT activation was determined by Western blot analysis using AKT phosphorylation-specific and total protein antibodies after the incubation of ChEC with the vehicle (con), NECA (10 µM), and combinations of NECA with caffeine, DPCPX (A 1 AR antagonist, 100 nM), or Istradefylline (A 2A AR antagonist, 1 µM) for 1 h. ( C ) Band intensities were assessed using the ImageJ software.
Strain Ncppb 1523, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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strain ncppb 1523 - by Bioz Stars, 2026-02
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nc and drp1-sirna-1203, -1523 and -2154 labeled with fam (green fluorescence)  (Shanghai GenePharma)
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Shanghai GenePharma nc and drp1-sirna-1203, -1523 and -2154 labeled with fam (green fluorescence)
The effects of AR antagonists on ChEC migration and AKT activation. ( A ) Cell migration was assessed using a Transwell assay in the presence of the vehicle (con), NECA (10 µM), or a combination of NECA with caffeine (400 µM), DPCPX (A 1 AR antagonist, 100 nM), Istradefylline (A 2A AR antagonist, 1 µM), MRS1754 (A 2B AR antagonist, 100 nM), or <t>MRS1523</t> (A 3 AR antagonist, 100 nM). Migration was quantified by counting the number of cells that migrated through the membrane in 8 high-power fields (×200; *** p < 0.001, * p < 0.05, n = 3). ( B ) AKT activation was determined by Western blot analysis using AKT phosphorylation-specific and total protein antibodies after the incubation of ChEC with the vehicle (con), NECA (10 µM), and combinations of NECA with caffeine, DPCPX (A 1 AR antagonist, 100 nM), or Istradefylline (A 2A AR antagonist, 1 µM) for 1 h. ( C ) Band intensities were assessed using the ImageJ software.
Nc And Drp1 Sirna 1203, 1523 And 2154 Labeled With Fam (Green Fluorescence), supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nc and drp1-sirna-1203, -1523 and -2154 labeled with fam (green fluorescence) - by Bioz Stars, 2026-02
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sirna-1523 labeled with fam  (Shanghai GenePharma)
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Shanghai GenePharma sirna-1523 labeled with fam
The effects of AR antagonists on ChEC migration and AKT activation. ( A ) Cell migration was assessed using a Transwell assay in the presence of the vehicle (con), NECA (10 µM), or a combination of NECA with caffeine (400 µM), DPCPX (A 1 AR antagonist, 100 nM), Istradefylline (A 2A AR antagonist, 1 µM), MRS1754 (A 2B AR antagonist, 100 nM), or <t>MRS1523</t> (A 3 AR antagonist, 100 nM). Migration was quantified by counting the number of cells that migrated through the membrane in 8 high-power fields (×200; *** p < 0.001, * p < 0.05, n = 3). ( B ) AKT activation was determined by Western blot analysis using AKT phosphorylation-specific and total protein antibodies after the incubation of ChEC with the vehicle (con), NECA (10 µM), and combinations of NECA with caffeine, DPCPX (A 1 AR antagonist, 100 nM), or Istradefylline (A 2A AR antagonist, 1 µM) for 1 h. ( C ) Band intensities were assessed using the ImageJ software.
Sirna 1523 Labeled With Fam, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aav type 5  (ATCC)
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ATCC aav type 5
The effects of AR antagonists on ChEC migration and AKT activation. ( A ) Cell migration was assessed using a Transwell assay in the presence of the vehicle (con), NECA (10 µM), or a combination of NECA with caffeine (400 µM), DPCPX (A 1 AR antagonist, 100 nM), Istradefylline (A 2A AR antagonist, 1 µM), MRS1754 (A 2B AR antagonist, 100 nM), or <t>MRS1523</t> (A 3 AR antagonist, 100 nM). Migration was quantified by counting the number of cells that migrated through the membrane in 8 high-power fields (×200; *** p < 0.001, * p < 0.05, n = 3). ( B ) AKT activation was determined by Western blot analysis using AKT phosphorylation-specific and total protein antibodies after the incubation of ChEC with the vehicle (con), NECA (10 µM), and combinations of NECA with caffeine, DPCPX (A 1 AR antagonist, 100 nM), or Istradefylline (A 2A AR antagonist, 1 µM) for 1 h. ( C ) Band intensities were assessed using the ImageJ software.
Aav Type 5, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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slit3 recombinant protein  (R&D Systems)
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R&D Systems slit3 recombinant protein
The effects of AR antagonists on ChEC migration and AKT activation. ( A ) Cell migration was assessed using a Transwell assay in the presence of the vehicle (con), NECA (10 µM), or a combination of NECA with caffeine (400 µM), DPCPX (A 1 AR antagonist, 100 nM), Istradefylline (A 2A AR antagonist, 1 µM), MRS1754 (A 2B AR antagonist, 100 nM), or <t>MRS1523</t> (A 3 AR antagonist, 100 nM). Migration was quantified by counting the number of cells that migrated through the membrane in 8 high-power fields (×200; *** p < 0.001, * p < 0.05, n = 3). ( B ) AKT activation was determined by Western blot analysis using AKT phosphorylation-specific and total protein antibodies after the incubation of ChEC with the vehicle (con), NECA (10 µM), and combinations of NECA with caffeine, DPCPX (A 1 AR antagonist, 100 nM), or Istradefylline (A 2A AR antagonist, 1 µM) for 1 h. ( C ) Band intensities were assessed using the ImageJ software.
Slit3 Recombinant Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hu54 ay530616 rh52 ay530565 virus 5 af085716 adeno  (ATCC)
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ATCC hu54 ay530616 rh52 ay530565 virus 5 af085716 adeno
The effects of AR antagonists on ChEC migration and AKT activation. ( A ) Cell migration was assessed using a Transwell assay in the presence of the vehicle (con), NECA (10 µM), or a combination of NECA with caffeine (400 µM), DPCPX (A 1 AR antagonist, 100 nM), Istradefylline (A 2A AR antagonist, 1 µM), MRS1754 (A 2B AR antagonist, 100 nM), or <t>MRS1523</t> (A 3 AR antagonist, 100 nM). Migration was quantified by counting the number of cells that migrated through the membrane in 8 high-power fields (×200; *** p < 0.001, * p < 0.05, n = 3). ( B ) AKT activation was determined by Western blot analysis using AKT phosphorylation-specific and total protein antibodies after the incubation of ChEC with the vehicle (con), NECA (10 µM), and combinations of NECA with caffeine, DPCPX (A 1 AR antagonist, 100 nM), or Istradefylline (A 2A AR antagonist, 1 µM) for 1 h. ( C ) Band intensities were assessed using the ImageJ software.
Hu54 Ay530616 Rh52 Ay530565 Virus 5 Af085716 Adeno, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hu54 ay530616 rh52 ay530565 virus 5 af085716 adeno associated nc 001862 hu7 ay530628 rh53 ay530566 virus 6 avian aav atcc ay186198  (ATCC)
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ATCC hu54 ay530616 rh52 ay530565 virus 5 af085716 adeno associated nc 001862 hu7 ay530628 rh53 ay530566 virus 6 avian aav atcc ay186198
The effects of AR antagonists on ChEC migration and AKT activation. ( A ) Cell migration was assessed using a Transwell assay in the presence of the vehicle (con), NECA (10 µM), or a combination of NECA with caffeine (400 µM), DPCPX (A 1 AR antagonist, 100 nM), Istradefylline (A 2A AR antagonist, 1 µM), MRS1754 (A 2B AR antagonist, 100 nM), or <t>MRS1523</t> (A 3 AR antagonist, 100 nM). Migration was quantified by counting the number of cells that migrated through the membrane in 8 high-power fields (×200; *** p < 0.001, * p < 0.05, n = 3). ( B ) AKT activation was determined by Western blot analysis using AKT phosphorylation-specific and total protein antibodies after the incubation of ChEC with the vehicle (con), NECA (10 µM), and combinations of NECA with caffeine, DPCPX (A 1 AR antagonist, 100 nM), or Istradefylline (A 2A AR antagonist, 1 µM) for 1 h. ( C ) Band intensities were assessed using the ImageJ software.
Hu54 Ay530616 Rh52 Ay530565 Virus 5 Af085716 Adeno Associated Nc 001862 Hu7 Ay530628 Rh53 Ay530566 Virus 6 Avian Aav Atcc Ay186198, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The effects of AR antagonists on ChEC migration and AKT activation. ( A ) Cell migration was assessed using a Transwell assay in the presence of the vehicle (con), NECA (10 µM), or a combination of NECA with caffeine (400 µM), DPCPX (A 1 AR antagonist, 100 nM), Istradefylline (A 2A AR antagonist, 1 µM), MRS1754 (A 2B AR antagonist, 100 nM), or MRS1523 (A 3 AR antagonist, 100 nM). Migration was quantified by counting the number of cells that migrated through the membrane in 8 high-power fields (×200; *** p < 0.001, * p < 0.05, n = 3). ( B ) AKT activation was determined by Western blot analysis using AKT phosphorylation-specific and total protein antibodies after the incubation of ChEC with the vehicle (con), NECA (10 µM), and combinations of NECA with caffeine, DPCPX (A 1 AR antagonist, 100 nM), or Istradefylline (A 2A AR antagonist, 1 µM) for 1 h. ( C ) Band intensities were assessed using the ImageJ software.

Journal: Cells

Article Title: Caffeine Mitigates Adenosine-Mediated Angiogenic Properties of Choroidal Endothelial Cells Through Antagonism of A 1 Adenosine Receptor and PI3K-AKT Axis

doi: 10.3390/cells15010087

Figure Lengend Snippet: The effects of AR antagonists on ChEC migration and AKT activation. ( A ) Cell migration was assessed using a Transwell assay in the presence of the vehicle (con), NECA (10 µM), or a combination of NECA with caffeine (400 µM), DPCPX (A 1 AR antagonist, 100 nM), Istradefylline (A 2A AR antagonist, 1 µM), MRS1754 (A 2B AR antagonist, 100 nM), or MRS1523 (A 3 AR antagonist, 100 nM). Migration was quantified by counting the number of cells that migrated through the membrane in 8 high-power fields (×200; *** p < 0.001, * p < 0.05, n = 3). ( B ) AKT activation was determined by Western blot analysis using AKT phosphorylation-specific and total protein antibodies after the incubation of ChEC with the vehicle (con), NECA (10 µM), and combinations of NECA with caffeine, DPCPX (A 1 AR antagonist, 100 nM), or Istradefylline (A 2A AR antagonist, 1 µM) for 1 h. ( C ) Band intensities were assessed using the ImageJ software.

Article Snippet: Caffeine citrate (51754-0500-1; Exela Pharma Sciences, Lenoir, NC, USA), Bz-ATP (2′(3′)-O-(4-benzoylbenzoyl) adenosine -5ʹ -triphosphate, tri(triethylammonium) salt (HY-136254), NECA (5′-N-ethylcarboxamidoadenosine, HY-103173), DPCPX (A 1 AR antagonist, HY-100937), Istradefylline (A 2A AR antagonist, HY-10888), MRS1754 (A 2B AR antagonist, HY-14121), MRS1523 (A 3 AR antagonist, HY-121119), SU5402 (VEGFR2 inhibitor, HY-10407) (Med Chem Express, Monmouth Junction, NJ, USA), LY294002 (PI3K inhibitor, L9908; Sigma), and AZ 11645373 (P2X7 receptor antagonist, 3317; R&D Systems, Minneapolis, MN, USA) were prepared with the desired concentration in EC growth medium.

Techniques: Migration, Activation Assay, Transwell Assay, Membrane, Western Blot, Phospho-proteomics, Incubation, Software

Effects of caffeine and A 1 , A 2A , A 2B , and A 3 AR antagonism on intracellular cAMP production in ChEC. Intracellular cAMP levels were assessed following treatment with the vehicle (con), NECA (10 µM), or a combination of NECA with caffeine (400 µM), DPCPX (100 nM; A 1 AR antagonist), Istradefylline (A 2A AR antagonist; 1 µM), MRS1754 (A 2B AR antagonist, 100 nM), or MRS1523 (A 3 AR antagonist, 100 nM). Antagonists were pre-treated for 10 min, followed by 10 min incubation with NECA (* p < 0.05, ** p < 0.01; n = 3).

Journal: Cells

Article Title: Caffeine Mitigates Adenosine-Mediated Angiogenic Properties of Choroidal Endothelial Cells Through Antagonism of A 1 Adenosine Receptor and PI3K-AKT Axis

doi: 10.3390/cells15010087

Figure Lengend Snippet: Effects of caffeine and A 1 , A 2A , A 2B , and A 3 AR antagonism on intracellular cAMP production in ChEC. Intracellular cAMP levels were assessed following treatment with the vehicle (con), NECA (10 µM), or a combination of NECA with caffeine (400 µM), DPCPX (100 nM; A 1 AR antagonist), Istradefylline (A 2A AR antagonist; 1 µM), MRS1754 (A 2B AR antagonist, 100 nM), or MRS1523 (A 3 AR antagonist, 100 nM). Antagonists were pre-treated for 10 min, followed by 10 min incubation with NECA (* p < 0.05, ** p < 0.01; n = 3).

Article Snippet: Caffeine citrate (51754-0500-1; Exela Pharma Sciences, Lenoir, NC, USA), Bz-ATP (2′(3′)-O-(4-benzoylbenzoyl) adenosine -5ʹ -triphosphate, tri(triethylammonium) salt (HY-136254), NECA (5′-N-ethylcarboxamidoadenosine, HY-103173), DPCPX (A 1 AR antagonist, HY-100937), Istradefylline (A 2A AR antagonist, HY-10888), MRS1754 (A 2B AR antagonist, HY-14121), MRS1523 (A 3 AR antagonist, HY-121119), SU5402 (VEGFR2 inhibitor, HY-10407) (Med Chem Express, Monmouth Junction, NJ, USA), LY294002 (PI3K inhibitor, L9908; Sigma), and AZ 11645373 (P2X7 receptor antagonist, 3317; R&D Systems, Minneapolis, MN, USA) were prepared with the desired concentration in EC growth medium.

Techniques: Incubation